… cryogenic isolation of therapeutic cells: novel approach for enzyme-free isolation of pancreatic islets using in situ cryopreservation of islets and concurrent selective …
MJ Taylor, SC Baicu - Cell transplantation, 2014 - journals.sagepub.com
… of selective acinar cell destruction was achieved. In contrast with conventional … enzyme-free
method of islet isolation relying on in situ cryopreservation of islets with simultaneous freeze …
method of islet isolation relying on in situ cryopreservation of islets with simultaneous freeze …
Cryopreservation: an overview of principles and cell-specific considerations
D Whaley, K Damyar, RP Witek… - Cell …, 2021 - journals.sagepub.com
… allows the banking of a large number of cells and tissues that … cryopreservation to maximize
post-cryo viability for each cell … freeze-thaw rates carries with it the potential to damage cells …
post-cryo viability for each cell … freeze-thaw rates carries with it the potential to damage cells …
Islet isolation from juvenile porcine pancreas after 24-h hypothermic machine perfusion preservation
MJ Taylor, S Baicu, E Greene, A Vazquez… - Cell …, 2010 - journals.sagepub.com
… examined islet isolation after 30 min WIT in situ without (G5, n … removal and freezing (0.5
ml), and islet resuspension in the … Figure 5C shows an intact islet surrounded by acinar tissue …
ml), and islet resuspension in the … Figure 5C shows an intact islet surrounded by acinar tissue …
In vitro maturation of viable islets from partially digested young pig pancreas
M Lamb, K Laugenour, O Liang… - Cell …, 2014 - journals.sagepub.com
… , storage at low temperature, and culture (47,51). … a progressive loss of acinar tissue
surrounding the islets, which … well demarcated in situ but fragile, resulting in substantial islet losses …
surrounding the islets, which … well demarcated in situ but fragile, resulting in substantial islet losses …
Characterization of integrin expression in islets isolated from hamster, canine, porcine, and human pancreas
RN Wang, S Paraskevas… - … of Histochemistry & …, 1999 - journals.sagepub.com
… , however, involves the dissociation of pancreatic tissue by a … on acinar, duct, and islet cells
in the normal pancreas of the … in situ and in isolated islets. Bars: A,B = 18 μm; C,D = 22 μm. …
in the normal pancreas of the … in situ and in isolated islets. Bars: A,B = 18 μm; C,D = 22 μm. …
Islet culture/preservation before islet transplantation
H Noguchi, C Miyagi-Shiohira, K Kurima… - Cell …, 2015 - journals.sagepub.com
… tissue (34). Harmful enzymes that are released from dying acinar cells during culture may be
deleterious to islet cell … (due to the low temperature), even if they are released by the dying …
deleterious to islet cell … (due to the low temperature), even if they are released by the dying …
Evolution of islet transplantation for the last 30 years
AC Farney, DER Sutherland, EC Opara - Pancreas, 2016 - journals.lww.com
… Because islet isolation requires manipulation of human tissue… therapeutic option for diabetes,
islet transplantation, as a cell … of nonenzyme-based procedures for islet isolation 146,147 is …
islet transplantation, as a cell … of nonenzyme-based procedures for islet isolation 146,147 is …
Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion
JRT Lakey, GL Warnock, AMJ Shapiro… - Cell …, 1999 - journals.sagepub.com
… pancreas is crucial to the subsequent ability to isolate viable … approach is to perfuse the
pancreas via the pancreatic duct … , the amount of undigested tissue remaining after dissociation …
pancreas via the pancreatic duct … , the amount of undigested tissue remaining after dissociation …
[HTML][HTML] Continuous quadrupole magnetic separation of islets during digestion improves purified porcine islet viability
BP Weegman, VS Kumar Sajja, TM Suszynski… - Journal of Diabetes …, 2016 - hindawi.com
… and selective destruction of acinar tissue,” Transplantation … , “Nonenzymatic cryogenic
isolation of therapeutic cells: novel approach for enzyme-free isolation of pancreatic islets using in …
isolation of therapeutic cells: novel approach for enzyme-free isolation of pancreatic islets using in …
Collagenase does not persist in human islets following isolation
SE Cross, SJ Hughes, A Clark, DWR Gray… - 2012 - journals.sagepub.com
… Tissue dissociation enzymes for islet isolation have been extensively … Tissue samples were
taken from the body of the pancreas immediately after collagenase infusion and snap frozen …
taken from the body of the pancreas immediately after collagenase infusion and snap frozen …